๐Ÿงช
Practical 4Spec ref: 4.2.2.2

Food Tests

Linked to B2: Organisation

Aim

Test food samples for the presence of glucose (reducing sugar), starch, protein and lipid.

Equipment

  • โ€ขTest tubes
  • โ€ขWater bath at 80ยฐC
  • โ€ขBenedict's solution
  • โ€ขBiuret reagent (NaOH + copper sulfate)
  • โ€ขIodine solution
  • โ€ขEthanol
  • โ€ขDistilled water
  • โ€ขFood samples

Variables

Independent variable
Type of food sample being tested
Dependent variable
Colour change in each test
Controlled variables
Volume of reagent, volume of food sample, temperature of water bath

Key Facts to Know

โœ“Reducing sugar (glucose): Benedict's โ†’ brick red/orange (heat required)
โœ“Starch: iodine solution โ†’ blue-black
โœ“Protein: biuret reagent โ†’ purple/violet
โœ“Lipid: ethanol emulsion test โ†’ milky white emulsion
โœ“Negative results: Benedict's = blue, iodine = orange-brown, biuret = blue, emulsion = clear

โš ๏ธ Common Mistakes

  • โœ—Getting positive result colours wrong โ€” must be exact (brick red, not just red)
  • โœ—Adding biuret reagents in wrong order โ€” NaOH must go in first
  • โœ—Not heating Benedict's test โ€” it won't change colour without heat

๐Ÿ’ก Exam Tips

  • โ˜…Learn exact positive colours for all four tests โ€” they are often tested
  • โ˜…State the reagent AND the colour change for full marks
  • โ˜…Benedict's needs heating โ€” lipid/starch/protein tests do not require heat